Journal: bioRxiv

Article Title: Chemokines form complex signals during inflammation and disease that can be decoded by extracellular matrix proteoglycans

doi: 10.1101/2022.09.20.508420

Figure Lengend Snippet: (A) CXCL9, 10 and 11 can all bind and signal through the chemokine receptor CXCR3 that is typically found on T cells. (B) The EMBL-ELI expression atlas (human) was analysed for relatedness in expression of CXCR3 and its’ ligands CXCL9, 10 and 11 across all tissues or (C) in distinct tissues and diseases.

Article Snippet: Specific concentrations of CXCL9 were measured by enzyme-linked immunosorbent assay (ELISA), using the mouse CXCL9 ELISA kit (R&D Systems) in a 96-well high binding ELISA plate following the manufacturer’s instructions.

Techniques: Expressing

Journal: bioRxiv

Article Title: Chemokines form complex signals during inflammation and disease that can be decoded by extracellular matrix proteoglycans

doi: 10.1101/2022.09.20.508420

Figure Lengend Snippet: (A) Schematic of the in vivo air pouch leukocyte recruitment model. (B) Analysis of chemokine concentration in the carrageenan inflamed air pouch. (C) Representative tSNE of all murine cells gated on live, single, CD45 + and built on CD4, CD8, F4/80, Ly6C, Ter119, CD3, TCRβ, CXCR3, Ly6G, CD11c, B220, CD11b, CD64, Siglec F, NK1.1 and TCRγδ. FlowSOM clusters are illustrated by gates. (D) tSNE analysis of air pouches injected with equimolar amounts of CXCL9, 10 and 11. (E) Quantification of all leukocytes (CD45 + ) and T cells within the air pouch following injection of CXCL9, 10 or 11. E analysed using a one-way ANOVA.

Article Snippet: Specific concentrations of CXCL9 were measured by enzyme-linked immunosorbent assay (ELISA), using the mouse CXCL9 ELISA kit (R&D Systems) in a 96-well high binding ELISA plate following the manufacturer’s instructions.

Techniques: In Vivo, Concentration Assay, Injection

Journal: bioRxiv

Article Title: Chemokines form complex signals during inflammation and disease that can be decoded by extracellular matrix proteoglycans

doi: 10.1101/2022.09.20.508420

Figure Lengend Snippet: (A) Schematic of HS GAG structure, including sulphation points and the enzymes that produce them. (B) Normalised (relative to wild type) binding of labelled CXCL9, 10 or 11 to genetically modified CHO cells. (C) Normalised and absolute binding of CXCL9, 10 and 11 to CHO cells in which KS^ST1/2/3 have been genetically removed. (D and E) Normalised binding of CXCL9, 10 and 11 to CHO cells genetically engineered to express the enzymes regulating 3-O GAG sulphation. (F) EMBL-ELI expression atlas analysis of relatednessCXCL9, 10 and 11 and GAG sulphation gene expression. B and D, data plotted as mean from three separate pooled experiments. C and E data plotted as mean ± SEM from three separate pooled experiments and analysed using a one-way ANOVA.

Article Snippet: Specific concentrations of CXCL9 were measured by enzyme-linked immunosorbent assay (ELISA), using the mouse CXCL9 ELISA kit (R&D Systems) in a 96-well high binding ELISA plate following the manufacturer’s instructions.

Techniques: Binding Assay, Genetically Modified, Expressing, Gene Expression

Journal: bioRxiv

Article Title: Chemokines form complex signals during inflammation and disease that can be decoded by extracellular matrix proteoglycans

doi: 10.1101/2022.09.20.508420

Figure Lengend Snippet: (A) CXCL9, 10 and 11 all bind to the same receptor with different affinities and biased signalling outcomes and are found in over-lapping expression patterns during inflammation and disease. (B) Differential GAG interactions means that CXCL9 is more likely to be retained on GAGs on the cell surface or within the ECM, with CXCL10 and CXCL11 being more likely to be present in their soluble state.

Article Snippet: Specific concentrations of CXCL9 were measured by enzyme-linked immunosorbent assay (ELISA), using the mouse CXCL9 ELISA kit (R&D Systems) in a 96-well high binding ELISA plate following the manufacturer’s instructions.

Techniques: Expressing

Journal: EBioMedicine

Article Title: Histone deacetylases inhibitor chidamide synergizes with humanized PD1 antibody to enhance T-cell chemokine expression and augment Ifn-γ response in NK-T cell lymphoma.

doi: 10.1016/j.ebiom.2022.104420

Figure Lengend Snippet: Fig. 5: Anti-PD1/chidamide shows an immune response with epigenetic regulation. a, Western blot of Ifn-γ, Cxcl9, Cxcr3, Acetylated histone H3, histone H3, Pd-l1, and β-actin in anti-PD1/chidamide and each monotherapy. b, Western blot of Ifn-γ, Cxcl9, Cxcr3, Acetylated histone H3,

Article Snippet: Tissue homogenates were detected in the Cxcl9 ELISA kit (CSB-EL006252MO, Cusabio) and Ifn-γ ELISA kit (KE10001, Proteintech).

Techniques: Western Blot